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Literature summary extracted from

  • Ulaganathan, T.; Shi, R.; Yao, D.; Gu, R.X.; Garron, M.L.; Cherney, M.; Tieleman, D.P.; Sterner, E.; Li, G.; Li, L.; Linhardt, R.J.; Cygler, M.
    Conformational flexibility of PL12 family heparinases structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657) (2017), Glycobiology, 27, 176-187 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.8
-
 Bacteroides thetaiotaomicron

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.8 structures of two crystal forms at resolution 1.8 and 2.4 A. Tyr301 is the main catalytic residue Bacteroides thetaiotaomicron

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.8 H431A complete loss of activity Bacteroides thetaiotaomicron
4.2.2.8 N247A complete loss of activity Bacteroides thetaiotaomicron
4.2.2.8 Y301F complete loss of activity Bacteroides thetaiotaomicron

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.8 0.593
-
 heparan sulfate pH 7.5, 23°C Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.8 Bacteroides thetaiotaomicron Q89YS4
-
-
4.2.2.8 Bacteroides thetaiotaomicron DSM 2079 Q89YS4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.8 desulfated N-sulfated heparosan
-
 Bacteroides thetaiotaomicron desulfated N-sulfated heparosan disaccharide
-
 ?
4.2.2.8 desulfated N-sulfated heparosan
-
 Bacteroides thetaiotaomicron DSM 2079 desulfated N-sulfated heparosan disaccharide
-
 ?
4.2.2.8 heparan sulfate
-
 Bacteroides thetaiotaomicron heparan disaccharide
-
 ?
4.2.2.8 heparan sulfate
-
 Bacteroides thetaiotaomicron DSM 2079 heparan disaccharide
-
 ?
4.2.2.8 heparin
-
 Bacteroides thetaiotaomicron heparin disaccharide
-
 ?
4.2.2.8 heparin
-
 Bacteroides thetaiotaomicron DSM 2079 heparin disaccharide
-
 ?
4.2.2.8 heparosan
-
 Bacteroides thetaiotaomicron heparosan disaccharide
-
 ?
4.2.2.8 heparosan
-
 Bacteroides thetaiotaomicron DSM 2079 heparosan disaccharide
-
 ?
4.2.2.8 N-desulfated, N-acetylated heparin
-
 Bacteroides thetaiotaomicron N-desulfated, N-acetylated heparin disaccharide
-
 ?
4.2.2.8 N-desulfated, N-acetylated heparin
-
 Bacteroides thetaiotaomicron DSM 2079 N-desulfated, N-acetylated heparin disaccharide
-
 ?
4.2.2.8 N-sulfoheparosan
-
 Bacteroides thetaiotaomicron N-sulfoheüparosan disaccharide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.8 BT4657
-
 Bacteroides thetaiotaomicron
4.2.2.8 HepIII
-
 Bacteroides thetaiotaomicron